1.
Etiological Study Of Pancytopenic Children
by Dr. Syed Maaz Nadeem | Dr. Muti-ur-Rehman Khan | Dr. Aftab Ahmed Anjum | Dr. Asim Aslam.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Pancytopenia is a hematological condition in which there is a decrease in all three cell lines of peripheral blood i.e. erythrocytes, leucocytes and platelets leading to anemia, leucopenia and thrombocytopenia. Complications of anemia, repeated infections and bleeding tendencies are sometimes horrifying and may result in death of individual.
The present study was designed to analyze the underlying pathology, different clinico-haematological features and importance of bone marrow study in one hundred children presenting with pancytopenia. Present study was carried out in pediatric laboratory of Mayo Hospital, Lahore, Pakistan..
Detailed history was taken in all cases. Complete blood counts were done on an automated blood analyzer (Sysmex Kx-21). For counter check of total leucocyte count, differential leucocyte count and platelets, smears were also prepared and stained by using Giemsa stain. Red cell morphology was done on blood smear for theconfirmation of red cell indices. A total volume of 3 ml venous blood was drawn into a syringe. Out of which 1.0 ml was delivered into EDTA containing vacutainer and remaining 2 ml blood was transferred to a plain glass tube. After clotting and centrifugation serum was obtained for screening of hepatitis B surface antigen and antibodies against hepatitis C virus. Bone marrow aspiration was also performed where indicated.
Megaloblastic anaemia (42%) Aplastic anaemia (26%) and ALL (8%) were found to be the common causes of pancytopenia in our scenario. Less common causes of pancytopenia were infections (8%), mixed deficiency (4%), MDS (4%) and lymphoma (4%). In all above mentioned cases clinical manifestations and peripheral blood counts played an important role in their evaluation.
Two cases of haemophagocytic syndrome (2%), a rare cause of pancytopenia were also diagnosed in this study.
This study also explained the importance of physical examination, peripheral blood findings and bone marrow examinations in the management of pancytopenic patients. Peripheral blood film and bone marrow aspiration should be performed simultaneously in pancytopenia patients when the diagnosis is not confirmed. Bone marrow examination in most cases gives the specific diagnosis. However, in few cases, additional tests may be required.
Serum vitamin B12 and folic acid levels may be needed for confirmation of megaloblastic anemia. Serum iron, TIBC and iron staining on bone marrow smears may be required in iron deficiency anemia. In cases of leukemia flow cytometry study may be more helpful in reaching a final diagnosis. Bone marrow biopsy is mandatory in aplastic anemia.
Availability: Items available for loan: UVAS Library [Call number: 1539,T] (1).
2.
Patho-Morphological Studies Of Reproductive Tract Disorders In Cattle And Buffalo
by Abdul Jabar | Dr. Ishtiaq Ahmed | Dr. Aftab Ahmed Anjum | Dr. Asim Aslam.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Reproductive tract disorders like endometritis, pyometra, follicular cysts, leuteal cysts etc have been reported as major causes of low conception rate and poor productivity of livestock. Reproductive tract lesions in cattle and buffaloes play an important role in animal breeding either by causing infertility or sterility of animals resulting in high economic losses. Besides of other reasons, presence of different types of bacteria inside the uterus of breeding animals causes deleterious effects on the reproductive tracts causing severe disorders and ultimately leads to failure of pregnancy. All these microbes results in the infection of uterus that ultimately affects animal's conception rate.
A total 100 reproductive tracts of slaughtered cattle (n=40) and buffaloes (n=60) were randomly collected from Lahore abattoir, Sherakot to study the impact of bacteria on the reproductive tracts, efficacy of variety of antibiotics on different bacterial isolates, gross and histopathological lesions. The swab samples for bacteriology were processed for purification and identification of bacterial isolates by doing number of conventional tests for final characterization and in vitro antibiotic sensitivity was performed. In this study seven different bacterial isolates were identified from all the samples. These include: Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Corynebacterium species, Aeromonas species, Micrococcus species and Lactobacillus species. By the analysis of in vitro antibiotic sensitivity, all eight different species of bacteria isolated were 100% sensitive to Ciprofloxacin, Norfloxacin and Chlortetracycline. Contrary to these, all bacteria were 100 % resistant to Bacitracin followed by Sulphaphenazole, Ticarcillin/Clavulanic acid and Trimethoprim.
Out of the total 100 reproductive tracts of cattle and buffaloes collected randomly, 54 showed lesions with different severity. Most of the lesions were uterine hemorrhages followed by pyometra, para-ovarian cysts, pyosalpinx, hydrosalpinx, leuteal cysts, follicular cysts and uterine tumors. These lesions were observed slightly higher in number in buffaloes than in cows. Tissue samples of reproductive tracts were processed for histopathology and histopathological lesions were observed under microscope at 4X, 10X and 40X.
Histopathological lesions in endometritis were hemorrhages in stratum compactum, very thick blood vessels, congestion, necrosis and degeneration of endometrial glands, glandular tissue fully filled with mononuclear cells, completely destroyed secretary epithelial cells. While in metritis, intact endometrial mucosa, proliferation of connective tissue, infilteration of inflammatory cells and mononuclear cells in the myometrium were observed.
In pyometra samples, thick layer of exudate on the surface of endometrium, severe infilteration of mononuclear cells, sloughing of endometrial mucosa, necrosis of endometrial glands and glands filled with heavy population of mononuclear cells, proliferation of fibrous connective tissues in between endometrial glands, many macrophages are also present, infiltration of polymorphs mononuclear cells were observed in stratum functionalis.
In uterine lymphoma, tumor tissue was in follicular pattern, neoplastic cells variable in size and having round nucleus, many mitotic figures present, and stroma consisted of broad irregular bands of collagen.
In conclusion, out of 100 reproductive tracts of slaughtered cattle and buffaloes were examined and gross lesions were found 54% including uterine hemorrhages 20% followed by pyometra, para-ovarian cysts, hydrosalpinx, luteal cysts, follicular cysts and uterine lymphoma as 17, 8, 3, 3, 2 and 1%, respectively. The bacteria isolated from reproductive tracts in cattle and buffaloes were Escherichia coli 100% followed by Staphylococcus aureus, Micrococcus species, Staphylococcus epidermidis, Lactobacillus species, Corynebacterium species and Aeromonas species as 51, 47, 40, 34, 24 and 5%, respectively. In endometritis, PMN cells, thickening of endometrium, congestion, very thick blood vessels, degeneration of endometrial glands, glandular tissue fully filled with mononuclear cells, completely destroyed secretary epithelial cells, intact endometrial mucosa, proliferation of connective tissue and hemorrhages are observed while in pyometra, sloughing of endometrial mucosa, glands filled with heavy population of mononuclear cells, proliferation of fibrous connective tissue in between endometrial glands, many macrophages are also present, congestion and degeneration of endometrial glands was seen. Tumor tissue was in follicular pattern, neoplastic cells variable in size and having round nucleus, many mitotic figures present, and stroma consisted of broad irregular bands of collagen.
Availability: Items available for loan: UVAS Library [Call number: 1541,T] (1).
3.
Pathogenesis Of Field Isolates Of Mannheimia Hemolytica In Experimentally Infected Rabbits
by Syeda Fakhra Waheed (2014-VA-10) | Prof. Dr. Zafar Iqbal Chaudhry | Prof. Dr. Asim Aslam | Prof. Dr. Aftab Ahmed Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Shipping fever is one of the most economically important infectious diseases of ruminants with a wide prevalence throughout the continents. The disease is characterized by an acute febrile course with severe fibrinous bronchopneumonia. Infected animals may die within a few days of the onset of clinical signs, but those which survive the acute attack may become chronically infected. Both Mannheimia and Pasteurella species are commensally resident in the respiratory tract of healthy ruminants and are capable of causing infection in animals with compromised pulmonary defense system. Bovine respiratory disease (BRD) is the most common and costly problem encountered in stocker or feedlot calves. BRD also called “shipping fever”, accounts for major economic losses to the producer by reducing average daily gain, feed efficiency, and overall performance of beef calves. The aim of present study was isolation of M.haemolytica from cattle. The identification of organism was performed through biochemical tests and confirmation by polymerase chain reaction. The nature of disease was evaluated through gross and microscopic lesions.
A total of 50 tissue samples (25 lungs and 25 pharynx) were collected from Punjab Agriculture and Meat company Lahore and brought to the Department of Pathology UVAS, Lahore and were analyzed for biochemical and molecular detection of M .haemolytica. For studying the pathogenesis of the disease, experimental infection was given to rabbits in Department of Pathology, UVAS Lahore. Rabbits were randomly divided into Group A, Group B and Group C with nine rabbits (n=9) in each group. Experimental infection of field isolated M. hemolytica was given intratrachealy to the rabbits. Rabbits of group A and B were infected with 0.5 mL bacterial inoculum having 103 and 106 CFU/mL respectively. The rabbits of Group C served as control group. Rectal temperature of each rabbit was recorded daily. On postmortem,
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gross and microscopic lesions were recorded.
The results showed that rabbits of control group not showed any gross or microscopic change. There was significant increase in rectal temperature of infected rabbits as compared to uninfected rabbits. The gross lesions were specific for the organism which was prominently observed in lungs of rabbits. The microscopic lesions revealed that there was severe consolidation, congestion and fibrin exudation in lungs of rabbits of group A which were given less number of organism and they developed clear signs of disease. The rabbits of Group B showed less prominent signs compared to group A due to early death of rabbits. There were multiple hemorrhages, of varying sizes and hyalinization of myocardial cells in infected rabbits. The severity of changes was significantly more different in Group A, as compared to Group B.
It can be deduced by this study that the rabbit can be used as a model for further studies exploring the pathogenesis of the disease as the lesions resemble to shipping fever caused by M. hemolytica in ruminants. The lesions, which developed, could be descending infection resulting in typical lesions of bronchopneumonia or lobular pneumonia. Availability: Items available for loan: UVAS Library [Call number: 2517-T] (1).
